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Biosensors in Arctic bivalves for environmental monitoring

Monitoring the quality of ocean water is important, for various societal, economic and ecological reasons. But it is also costly and time-consuming, and the monitoring task itself can sometimes put humans at risk. We are looking for better ways to monitor water quality in high-risk environments.

By: Hector Andrade and Lionel Camus // Akvaplan-niva and Damien Tran and Pierre Ciret // CNRS and University of Bordeaux, UMR EPOC 5805, Arcachon, France

Traditional systems for monitoring water quality in aquatic environments are rather expensive and rely on intensive exploitation of human resources to collect samples, do chemical analysis, and measure toxicity. This is especially true in the Arctic, where environmental conditions can be harsh, particularly in the winter due to low temperatures and poor availability of light. A desirable solution is to develop systems that can work without hands-on human control, either as sensors by themselves or as early warning detectors to trigger a sampling campaign.

In 2012, researchers from Akvaplan-niva AS and EPOC, a research unit of the University of Bordeaux and the French National Centre for Scientific Research, teamed up to deploy a valvometer – a device that monitors the gaping behavior of bivalves – in Ny-Ålesund, Svalbard. The aim of this deployment was to adapt this biosensor developed by EPOC to Arctic conditions in order to study how the Icelandic scallop Chlamys islandica behaves and how its biologic rhythms are adapted to the Arctic environment. A new deployment with blue mussels Mytilus sp. was carried out in 2016 to study that species’ biology. The blue mussel had disappeared from Svalbard, but due to climate warming, it has resettled since the beginning of the 2000s.

To monitor the bivalves’ activity directly in the field, the valvometer is installed inside a cage. A pair of extremely light electrodes with flexible cables are glued on each half shell. An electromagnetic current generated between the electrodes makes it possible to measure the mollusk shell opening and closing. In a typical biosensor deployment, sixteen pairs of electrodes are coupled to a waterproof box next to the animals, simultaneously recording the behavior of sixteen bivalves.

Damien Tran gluing sensors to Icelandic scallops in the lab. Photo: Carl Ballantine

 

This box contains a circuit board that manages the electrodes and is connected by cable to a second circuit board at the sea surface or on land. The data are saved and digitised, and then transferred to a supercomputer. The system measures the bivalves’ opening status every 1.6 seconds.

The continuous monitoring of Icelandic scallops and, more recently, blue mussels in Ny-Ålesund is allowing us to understand the baseline gaping behavior and shell growth of both species and how changes in the environment affect the species’ life cycles. A surprising discovery was that despite the lack of light during the polar night, both species continue to grow in winter, and maintain a daily cycle in their behavior.

Once we have characterised baseline behavior, we can study how stressors such as increased temperature or the presence of toxins in the water disturb the species’ gaping behavior. In 2020, we will expose Icelandic scallops to harmful algae under laboratory conditions, to test whether the species changes its baseline behavior. If we manage to record behavioral changes that can be attributed to the presence of phycotoxins in the water, we will have developed a biosensor that can be employed as an early warning system to detect harmful algae blooms in the north. Such monitoring tools are much-needed nowadays, especially by the salmon aquaculture industry.

Future projects also include testing whether the biosensor coupled with a hydrophone can be employed to study the effects of noise pollution in the sea. In this regard, this innovative tool holds promise for marine monitoring, allowing managers to assess the environmental status of marine and freshwater ecosystems remotely and in real-time.

Hector Andrade preparing an exposure experiment to test the biosensor. Photo: Lionel Camus

Icelandic scallops are ready to be deployed in the field with the electrodes glued onto their shells. Photos: Damien Tran

Further reading

Andrade H, Massabuau J-C, Cochrane S, Ciret P, Tran D, Sow M, Camus L (2016).High Frequency Non-invasive (HFNI) Bio-Sensors as a potential tool for marine monitoring and assessments. Frontiers in Marine Science 3.

Tran D, Sow M, Camus L, Ciret P, Berge J, Massabuau J-C (2016).In the darkness of the polar night, scallops keep on a steady rhythm. Scientific Reports 6, 32435.

This article was produced for Fram Forum 2020. Fram Forum is published by Framsenteret AS on behalf of FRAM – the High North Research Centre for Climate and the Environment.